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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION,10.1139/o59-099,Biochemistry and Cell Biology-biochimie Et Biologie Cellulaire,E. G. Bligh,W

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION   (Citations: 8037)
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    • ...Analysis of fatty acid composition in plasma cholesterol esters (CEs) was as follows: total lipid was extracted from plasma by using the method of Bligh and Dyer [26], followed by separation of the CEs by thin-layer chromatography using silica gel plates (Silica Gel 60, Merck, Darmstadt, Germany) and a solvent system of petroleum ether/ethyl ether/acetic acid (80:20:1, v/v/v)...

    Toshihide Shimaet al. Efficacy of long-term ezetimibe therapy in patients with nonalcoholic ...

    • ...The most common approach is a macro-gravimetric method in which lipids are extracted from a sample, the extraction solvent is evaporated and the retained material is measured as the lipid content [8, 9]. This traditional gravimetrical method requires a relatively large quantity of sample and is time-consuming and labor-intensive when analysis of many samples is needed...

    Yu-Shen Chenget al. Rapid Quantitative Analysis of Lipids Using a Colorimetric Method in a...

    • ...The most common is total lipid extraction with a Bligh and Dyer procedure [43], followed by separation on a Thin Layer Chromatography (TLC), recovery of the triacylglycerol (TAG) from the silica, transmethylation and quantification of the fatty acid methyl esters (FAMEs) by Gas Chromatography with Flame Ionization Detector (GC-FID)...
    • ...Extraction of total cellular lipids Lipids were extracted from C. reinhardtii cells by modified Bligh and Dyer [43]...

    Magali Siautet al. Oil accumulation in the model green alga Chlamydomonas reinhardtii: ch...

    • ...Lipid and fatty acids were analysed according to Rainuzzo et al. (1992) using a modified method of Bligh and Dyer (1959) for extraction of lipid and a derived method from Metcalfe et al. (1966) for fatty acid methyl esters (FAMEs)...

    Huy Quang NguyenHelgeet al. Effects of early weaning strategies on growth, survival and digestive ...

    • ...Tissue lipid measurement The purification of diacylglycerol from liver was performed as previously described [28, 29]...
    • ...Triacylglycerol was extracted using the method of Bligh and Dyer [29] and measured with a commercially available kit (DCL Triglyceride Reagent; Diagnostic Chemicals, Oxford, CT, USA)...

    D. Weismannet al. Knockdown of the gene encoding Drosophila tribbles homologue 3 ( Trib3...

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